MSc Courses

Program of Plant Physiology Subjects

The subject considers the basic plant physiological knowledge of various BSc-courses, and deals with several topics of plant growth and development. These include the water relation of plants (water potential and its importance, water balance of plants); plant and inorganic nutrients (essential elements, deficiency symptoms); nitrogen assimilation; photosynthesis (characteristics of light and carbon reduction phases, effect of environmental factors); occurrence, structure, biosynthesis, transport, metabolism and function of plant growth regulators such as auxin, cytokinins, gibberellins, other plant hormones; plant treatments by plant growth regulating substances; plant and biotic environment relationship; physiology of sick plant; plant stress physiology.


Program of Plant Biotechnology Subjects

The concept of plant biotechnology, topics, aims, methods and history. Plant somatic cell cultures, callus cultures. Plant regeneration (morphogenesis): physiological basics, organogenesis, somatic embryogenesis. Plant protoplast cultures: protoplast isolation and culture. Somatic hybridization: protoplast fusion, hybrid cells, identifying of plants. Isolation of mutants in cell and tissue cultures. Gametoclonal and somaclonal variability. Significance of plant gene technology. Molecular structure of plant genes. Identification of plant genes, genome maps. Recombinant DNA technology. In vitro recombination. Identification of plant genes. Gene cloning, clone library, clone selection. Gene transfer systems. Steps of transgenic plant production. Gene transfer methods for plants. Plant biotechnology and plant breeding: biotechnology of sexual reproduction (haploid cultures: ovary and ovulum cultures, microspore cultures, in vitro pollination and fertilization, triploid cultures), embryo cultures (in vivo embryo development, in vitro embryo development: pre-germinal and post-germinal cultures), culture of vegetative organs (root cultures, leaf cultures, meristem cultures). Plant biotechnology and breeding material production: in vitro vegetative micropropagation, artificial (synthetic) seed technology, pathogen free plants and plant biotechnology, in vitro gene bank. Plant biotechnology and crop protection: pathogen-resistant transgenic crops, pest resistant transgenic crops, herbicide-tolerant transgenic plants. GM crops in agriculture: advantages and disadvantages. Risk factors of plant gene technology. Legislative regulation of plant gene technology. Economic importance of GM crops.



Name of the subject


Plant nutrition and metabolic physiology


Applied plant physiology


Applied plant physiology


Applied plant physiology



Plant biotechnology


Plant biotechnology


Plant biotechnology


Optional subject:


Plant physiology