Plant Biotechnology

The Department Group of Plant Biology has been dealing with in vitro plant cell and tissue cultures since 1986. Dozens of the MACC microalgae strains proved to have significant plant hormone (auxin, cytokinin) production thus they may be suitable to influence the growth and development of tissue culture propagated plants.

According to our experimental results supported by several research funds, extracellular products or biomass of microalgae added to tissue culture media facilitated the callus development and the formation of shoots and roots in tobacco and peas. In recent years, we proved that in case of fodder beet, the medium supplemented with microalgae suspension supernatant stimulated root growth. Our goal in this field was the additional analysis of further strains of our microalgae collection on cell and tissue cultures of plant species which are difficult to be cultivated in vitro.

The large-seeded grain legumes (beans, peas, fenugreek) are among species which are difficult to be cultured in vitro (recalcitrant). To improve traditional breeding techniques with biotechnological applications is also required for these plants. Our research goals: to initiate in vitro tissue cultures on the species mentioned above together with the Beta vulgaris L. species, and maintain or modify them genetically and then regenerate plants.

Our research topics:

  • induction and maintenance of in vitro somatic cell and tissue cultures;
  • study of the effect of different culture conditions in the previously mentioned cultures, particularly the plant hormones of the media components;
  • examination of plant regeneration capability of in vitro cultures;
  • genetic modification of pea and Beta vulgaris L. in vitro tissue cultures by direct gene transfer method (biolistics).